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Job title:
• Laboratory Research Scientist
Closing date and time:
• 21 May 2021 at 23:59
Reports to:
• Paola Scaffidi
Contract term:
• This is a full-time, fixed term (12 month) position on Crick terms and conditions of employment.
Salary for this Role:
• Competitive with benefits, subject to skills and experience
Context and the research group
The Francis Crick Institute is a world-leading centre of biomedical research and innovation. It promotes connections between researchers and disciplines and between academic institutions, healthcare organisations and businesses. Dedicated to research excellence, the institute has the scale, vision and expertise to tackle the most challenging scientific questions underpinning health and disease. It is world-class with a strong national role—training scientists and developing ideas for public good.
Lab web page: https://www.crick.ac.uk/research/a-z-researchers/researchers-p-s/paola-scaffidi/
Project Summary
This 12-month Laboratory Research Scientist position will support research on epigenetic mechanisms important for cancer development within the Scaffidi Laboratory.
Key Responsibilities
• To ensure continuation of an ongoing project involving analysis of mouse embryos depleted of the linker histone H1.0. We have shown that H1.0 is an important inhibitor of cancer progression in numerous cancer type (Torres at al. Science 2016, Morales et al. Nature Comms 2020). We have recently found that H1.0-deficient mice show a strong embryonic lethal phenotype associated with cell hyperproliferation and we are characterizing the molecular basis of this effect. The project involves analysis of embryos by immunofluorescence microscopy using established protocols, and molecular characterisation of embryonic stem cells that enable temporal control of H1.0 depletion.
• To independently manage relevant mouse colonies
Key Experiences and Competencies
The post holder should embody and demonstrate our core Crick values: Bold, Imaginative, Open, Dynamic and Collegial, in addition to the following:
Essential
• BSc in Biochemistry, Cell Biology or related disciplines, or at least 5yrs of relevant laboratory experience.
• Experience in basic laboratory techniques such as cell culture, PCR, genomic DNA extraction, qRT-PCR, microscopy
Desirable:
• Highly desirable: Experience with animal work (mice)
• Experience with confocal microscopy
• PhD in a relevant discipline
• Experience working with GM mice
• Experience with the use of databases
• Experience with animal tissue handling (collection, dissociation, preparation of FFPE sections or cryosections)
• Holder of UK Animal scientific procedures personal license
• Experience with mouse embryonic stem cells
The post holder will be trained in all specific techniques by a Principal Laboratory Scientist and will receive support by the rest of the lab and the Crick core facilities.
If you are interested in applying for this role, please upload an up-to-date CV and a cover letter, explaining your motivation and suitability for this role.
Informal enquiries relating to this role can be addressed to Paola Scaffidi: Paola.Scaffidi@crick.ac.uk
